Article : Production And Partial Purification Of Microbial Enzymes Cellulase And Laccase Using A Laboratory Designed Solid State Fermenter

Title

Production And Partial Purification Of Microbial Enzymes Cellulase And Laccase Using A Laboratory Designed Solid State Fermenter

Author

Monica Attri, Arora Maninder, Loveleen Kaur Sarao

Utilization of agricultural waste (rice straw as substrate) through solid state fermentation by Trichoderma chrysosporium and Phanerochaete chrysosporium is an economical method of enzyme production. The present study was undertaken with a view to produce cellulase and laccase enzymes with enhanced enzyme activity using rice straw as a substrate by solid state fermentation. Fungal cellulase and laccase were produced by using specific cultures of Trichoderma chrysosporium and Phanerochaete chrysosporium respectively. For maximum enzyme production the optimum temperature, humidity and spore density were 30°C, 95% and 1x107 spores ml-1. With these optimum conditions enzyme activities in the range of cellulase (704.05U) and laccase (54.4IU) were obtained.

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