A high performance liquid chromatography method has been developed and validated for the analysis of Eugenol, a volatile flavoring agent, in Human plasma. Method involves the addition of 20-100 μg/ml concentrations of Eugenol to the plasma, extraction with methanol, removal of the protein precipitate, and analysis of the supernatant by reversed-phase (Cl8) HPLC using an methanol-water mixture (85:15 v/v) with UV detection at 280 nm. The calibration curve was linear with co-relation coefficient (r2) 0.9996. The precision, accuracy, sensitivity, and specificity of the method were assessed. The method was also applied to determine the concentration of Eugenol in the marketed formulation.
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