Article : In Vitro Propagation Of Limnophila Aromatica (Lam.) Merr., An Ornamental And Medicinal Aquatic Plant

Title

In Vitro Propagation Of Limnophila Aromatica (Lam.) Merr., An Ornamental And Medicinal Aquatic Plant

Author

Sheeja George E , Alphi Korath, Aney Kutty Joseph

Limnophila aromatica (Lam.) Merr., belonging to the family Scrophulariaceae, is a popular ornamental aquatic plant having great demands in the aquarium industry. The plant is appreciated for its medicinal importance too. In spite of its growing demands, the plant is least available in India. Therefore tissue culture techniques can serve as a powerful tool for the commercial production of this plant. An efficient protocol for the micropropagation of L. aromatica through multiple shoot proliferation from the nodal explants was developed successfully. Treating the nodal explants with 15% (v/v) commercial bleach (Robin liquid bleach, Reckitt Benckiser, India) for 10 minutes followed by a quick dip in 70% ethanol proved to be the best sterilization procedure to obtain axenic cultures. The aseptic nodal segments produced shoots within a week when cultured in a medium containing full strength Murashige and Skoog (MS) inorganic salts, 100 mg/l myo-inositol, 0.1 mg/l thiamine-HCl, 3% sucrose, 0.8% agar as gelling agent and supplemented with 0.5 to 2.5 with mgl-1 6- Benzyl aminopurine (BAP) alone or in combination with 0.1 or 0.2 mgl-1 α- Naphthalene acetic acid (NAA). Maximum number of shoots (5.13 shoots per explant, p˂ 0.05) was formed on MS medium supplemented with a combination of 2.0 mgl-1 BAP and 0.1 mgl-1 NAA. Maximum shoot length (2.69 cm, p˂ 0.05) and maximum number of roots were obtained on MS medium containing 0.5 mgl-1 BAP and 0.1 mgl-1 NAA. Rooted plantlets were successfully acclimatized and transferred to aquarium 

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